Problems in digital microscopy
نویسنده
چکیده
Three c ase studies are used to illustrate some issues which arise when digital image analysis methods are applied in microscopy. An algorithm is described for estimating the diameters of cashmere bres from out-of-focus images. The process of image formation in diierential interference c ontrast DIC microscopy is modelled, and used t o c onstruct templates for identifying and measuring algal, bacterial and fungal cells. Finally, the three-dimensional structure o f a diatom is recovered by analysing images from a series of focal planes.
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